Tumor | Treatment | Nr. of cells injected | Freq. SCs/CICs | 95% CI | P‐value | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|

10^{5} | 10^{4} | 5 × 10^{3} | 10^{3} | 10^{2} | 10^{1} | ||||||

Outgrowths/injections | |||||||||||

Numb^{−} tumors | T3 | Ctr | 2/2 | 4/4 | 3/4 | 2/8 | 0/8 | 0/4 | 1:3,290 | 1:1,550–1:6,985 | 0.013 |

T3 | Numb | 2/2 | 3/6 | 2/6 | 0/2 | 0/2 | 0/2 | 1:13,999 | 1:5,803–1:33,772 | ||

T4 | Ctr | 2/2 | 4/4 | 3/4 | 1/8 | 0/8 | 0/4 | 1:3,954 | 1:1,857–1:8,420 | 0.017 | |

T4 | Numb | 2/2 | 4/8 | 2/8 | 0/2 | 0/2 | 0/2 | 1:15,751 | 1:7,110–1:34,892 | ||

Numb^{+} tumors | TC | Ctr | 2/2 | 2/2 | 5/7 | 0/8 | 0/2 | 0/2 | 1:5,090 | 1:2,396–1:10,811 | 0.949 |

TC | Numb | 2/2 | 2/2 | 3/5 | 1/6 | 0/8 | 0/4 | 1:4,897 | 1:2,116–1:11,335 | ||

TD | Ctr | 2/2 | 2/2 | 4/6 | 1/8 | 0/2 | 0/2 | 1:4,660 | 1:2,147–1:10,115 | 0.649 | |

TD | Numb | 2/2 | 2/2 | 3/6 | 1/8 | 0/2 | 0/2 | 1:6,096 | 1:2,685–1:13,839 |

Numb

^{−}or Numb^{+}MECs, reconstituted with Numb‐DsRed (Numb) or mock‐infected (Ctr), were orthotopically transplanted at limiting dilutions in NGS mice. The number of outgrowths per number of injected pads (Outgrowths/injections) is indicated. The complete statistical analysis of the experiment, with the frequency of CICs and with 95% confidence intervals (CI), is shown. The frequencies were calculated by Poisson statistics, using the “StatMod” software package for the R computing environment (http://www.R-project.org), as previously described (Shackleton*et al*, 2006), and a complementary log‐log generalized linear model (two‐sided 95% Wald confidence intervals or, in case of zero outgrowths, one‐sided 95% Clopper–Pearson intervals). The single‐hit assumption was tested as recommended and was not rejected for any dilution series (*P*>