Table 1. Most abundant and selected proteins identified from samples prepared with solvent‐based, MonoPrep (top), and urea‐based standard (bottom) methods
MONOPREPAccessionProtein names# peptides identifiedMw (KDa)Abundance score (intensity/Mw)Abundance rankExpressed in Microarrays
10 most abundant proteinsP06454Prothymosin alpha512.1911.24E + 041
P62805Histone H4311.3541.04E + 042
P6160410 kDa heat shock protein, mitochondrial (Hsp10)410.9196.00E + 033
P02766Transthyretin (ATTR) (prealbumin) (TBPA)215.8694.02E + 035
P09936Ubiquitin carboxyl‐terminal hydrolase isozyme L1424.7962.94E + 036
P56279T‐cell leukemia/lymphoma protein 1A213.4442.89E + 037
Q14764Major vault protein (MVP)3299.2142.65E + 038
A6NGQ2Oocyte‐expressed protein homolog (OOEP)517.152.21E + 039
Q08257Quinone oxidoreductase435.1661.85E + 0310
P04406Glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH)436.0121.67E + 0311
Selected proteinsP06454Prothymosin alpha512.1911.24E + 041
A6NGQ2Oocyte‐expressed protein homolog (OOEP)517.152.21E + 039
P04406Glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH)436.0121.67E + 0311
Q587J8KHDC3‐like protein (ECAT1)224.2781.46E + 0313
Q99497Protein DJ‐1119.8688.06E + 0216
P00441Superoxide dismutase [Cu‐Zn]115.9187.46E + 0218
P16949Stathmin217.2834.05E + 0227
P0DJG4Testicular haploid expressed gene protein‐like152.9683.80E + 0231 a
Q9H808Transducin‐like enhancer protein 6 (TLE6)449.7712.19E + 0253
P14625Endoplasmin (heat shock protein 90 kDa beta member 1)692.3651.90E + 0256
P06576ATP synthase subunit beta, mitochondrial256.4951.90E + 0257
P04179Superoxide dismutase [Mn], mitochondrial124.6941.26E + 0272
P59047NACHT, LRR, and PYD domains‐containing protein 5 (Mater)5134.1871.11E + 0280
Q8WX94NACHT, LRR, and PYD domains‐containing protein 74111.6783.86E + 01111
Q9NX02NACHT, LRR, and PYD domains‐containing protein 21120.3768.94E + 00135
UREAAccessionProtein names# peptides identifiedMw (KDa)Abundance score (intensity/Mw)Abundance rankExpressed in Microarrays
10 most abundant proteinsQ01469Fatty acid‐binding protein, epidermal915.1479.24E + 055
P47929Galectin‐7715.0585.45E + 0510
P22528Cornifin‐B39.8763.44E + 0516
P10599Thioredoxin611.7242.99E + 0518
P01040Cystatin‐A410.9942.36E + 0520
P04792Heat shock protein beta‐1822.7572.02E + 0522
P55000Secreted Ly‐6/uPAR‐related protein 1 (SLURP‐1)311.1731.70E + 0524
P15090Fatty acid‐binding protein, adipocyte514.7021.29E + 0526
Q06830Peroxiredoxin‐1622.0859.51E + 0428
P04406Glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH)1136.0128.89E + 0429
Selected proteinsQ06830Peroxiredoxin‐1622.0859.51E + 0428
P04406Glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH)1136.0128.89E + 0429
P06702Protein S100‐A9 (calgranulin B)313.2274.76E + 0436
P05109Protein S100‐A8 (calgranulin A)310.8221.57E + 0459
Q8WVV4Protein POF1B (premature ovarian failure protein 1B)1367.9891.34E + 0461
P17931Galectin‐3326.1239.19E + 0371
P14174Macrophage migration inhibitory factor (MIF)112.4623.53E + 0396
Q99497Protein DJ‐1119.8682.97E + 03101
Q9NZH8Interleukin‐36 gamma (IL‐1 epsilon)118.72.78E + 03104
P28799Granulins263.4721.12E + 03134
  • Proteins identified in blank samples were considered as common contaminants and removed from the catalogue of identified proteins obtained from blastosol samples. The abundance ranking column refers to the relative protein intensity levels prior to exclusion of common contaminants. The proteins detected using the urea‐based method show less continuous numbering of ‘abundance rank’ because a relatively large number of high abundance contaminants were detected and excluded.

  • a Gene not present in Illumina HT12_v4 Microarrays platform.