The combinations of genetic alterations that cooperate with von Hippel–Lindau (VHL) mutation to cause clear cell renal cell carcinoma (ccRCC) remain poorly understood. We show that the TP53 tumour suppressor gene is mutated in approximately 9% of human ccRCCs. Combined deletion of Vhl and Trp53 in primary mouse embryo fibroblasts causes proliferative dysregulation and high rates of aneuploidy. Deletion of these genes in the epithelium of the kidney induces the formation of simple cysts, atypical cysts and neoplasms, and deletion in the epithelia of the genital urinary tract leads to dysplasia and tumour formation. Kidney cysts display a reduced frequency of primary cilia and atypical cysts and neoplasms exhibit a pro‐proliferative signature including activation of mTORC1 and high expression of Myc, mimicking several cellular and molecular alterations seen in human ccRCC and its precursor lesions. As the majority of ccRCC is associated with functional inactivation of VHL, our findings suggest that for a subset of ccRCC, loss of p53 function represents a critical event in tumour development.
The paper explained
The cooperating genetic events that lead to the formation of clear cell renal cell carcinoma (ccRCC), the most frequent form of kidney cancer in humans, remain unclear. While the vast majority of familial and sporadic forms of ccRCC harbour biallelic inactivation of the von Hippel–Lindau tumour suppressor gene (VHL), loss of VHL function alone in humans and in mice is insufficient to cause kidney tumour formation. It is presumed that other genetic events must cooperate with loss of VHL to cause ccRCC but these cooperating mutations remain poorly understood.
Here we identify loss of function mutations in TP53 in a subset of sporadic human ccRCCs and show that kidney‐specific combined deletion of Vhl and Trp53 leads to the formation of cysts and tumours in mice, recapitulating the precursor lesions and cellular and molecular alterations that are involved in the formation of VHL mutant ccRCC in humans.
These findings provide the first demonstration that secondary genetic alterations can cooperate with loss of VHL to cause kidney tumour formation and implicate TP53 mutations in the pathogenesis of a subset of human ccRCC.
Clear cell renal cell carcinoma (ccRCC) accounts for approximately 80% of kidney tumours and thereby approximately 2.5% of all types of human malignancy. The von Hippel–Lindau (VHL) tumour suppressor gene is mutated, deleted or epigenetically silenced in up to 85% of all sporadic cases of ccRCC (Maher, 2013). Germline inheritance of a single mutant allele of VHL gives rise to the dominantly inherited VHL familial cancer syndrome which predisposes not only to the formation of ccRCC, but also to cystic lesions in the kidney and pancreas as well as to diverse types of tumours in the central nervous system, eye, ear, pancreas, adrenal gland, epididymis and broad ligament (Kaelin, 2002).
The pVHL protein has been ascribed several distinct biochemical activities and implicated in the regulation of diverse cellular processes, dysregulation of any or all of which could be envisaged to play important roles in tumour formation (Frew & Krek, 2007). Two lines of evidence however suggest that loss of pVHL function alone is insufficient for tumour initiation in the kidney. Kidneys of patients with an inherited VHL mutation frequently display cystic lesions as well as ccRCC. Since some pVHL‐deficient proliferative cysts contain micro‐foci of ccRCC, it is believed that, at least in some cases, cysts represent a precursor lesion in the evolution of malignant ccRCC (Lubensky et al, 1996; Walther et al, 1995). Detailed analysis of regions of normal histology in these kidneys revealed that VHL patient kidneys likely contain many thousands of individual isolated cells that are null for pVHL function (Mandriota et al, 2002; Montani et al, 2010). pVHL‐deficient cysts and ccRCC apparently arise infrequently in comparison to the total frequency of VHL mutation. Secondly, heterozygous deletion of the mouse homologue of the VHL gene, Vhl (previously referred to as Vhlh), in the entire mouse (Haase et al, 2001), or homozygous deletion under the control of kidney‐specific Cre transgenes, does not lead to proliferative dysregulation or tumour formation in the kidney (Frew et al, 2008b; Rankin et al, 2006). Multiple genetic mutations appear to be required to cause proliferation and transformation of pVHL‐deficient cells.
Genes that are mutated at high frequency in diverse human epithelial tumours, including PTEN, EGFR, ERBB2, BRAF, RAS family genes, RB1 and APC, are either not mutated or are mutated at relatively low frequencies (<10%) in ccRCC. Rather, ccRCC frequently (41%) harbour mutations in the chromatin remodelling gene PBRM1 (Varela et al, 2011) and in several genes involved in histone modification (Dalgliesh et al, 2010) and protein ubiquitination and de‐ubiquitination (Guo et al, 2012; Pena‐Llopis et al, 2012). Several chromosomal regions are frequently amplified or deleted and numerous genes are frequently hypermethylated in ccRCC (Maher, 2013), implying that there may be many different combinations of genetic alterations that can cooperate with loss of VHL function to cause tumour formation. Our previous studies demonstrate that low‐frequency mutations could be functionally important in ccRCC formation; co‐deletion of Vhl and Pten in the mouse kidney led to the formation of proliferative cysts, mimicking the precursor lesions of ccRCC that arise in human VHL patients (Frew et al, 2008b). Several studies, including data presented herein, have shown that TP53 is mutated in a subset of ccRCC (http://cancer.sanger.ac.uk/cosmic). We demonstrate that combined mutation of Vhl and Trp53 causes dysregulation of cellular proliferation in primary mouse embryo fibroblasts (MEFs) and kidney epithelial cells and results in the formation of kidney cysts and neoplastic lesions in kidneys as well as tumours in genital tract organs.
TP53 mutations occur in sporadic ccRCCs
We sequenced the entire VHL gene and exons 5–8 of the TP53 gene in 54 cases of sporadic ccRCC (Table 1). As expected, missense or truncating VHL mutations were observed in 73% of the tumours. Immunohistochemistry for the HIF1α‐inducible proteins CA9 and Glut1, and for HIF1α itself, revealed moderate or strong expression of at least one of these markers in all but two of the tumours, verifying the well‐described hypoxic signature associated with loss of function of pVHL. TP53 mutations that affected the coding region were detected in 5 (9%) tumours, all of which are either previously described pathogenic mutations or are predicted to be pathogenic. One tumour harboured both VHL and TP53 mutations, while the other four TP53 mutant tumours were wild‐type for VHL. While methylation analyses for the VHL gene were not possible in these samples, it is likely that pVHL expression may be silenced in these tumours as they showed very high immunohistochemical staining for the HIFα target genes. In agreement with our data, the COSMIC database (http://cancer.sanger.ac.uk/cosmic) lists 30 of 209 (14.4%) tumours that display TP53 coding region mutations. Unfortunately the VHL mutation status of these tumours is in most cases unknown. Thus, TP53 is mutated in a significant fraction of sporadic ccRCCs.
Trp53 mutation allows immortalisation of Vhl mutant primary mouse embryo fibroblasts
We first utilized primary MEFs to investigate potential cooperative interactions of the mouse Vhl and Trp53 genes in proliferative control. Deletion of Vhl in primary and transformed MEFs induces proliferation arrest and/or the onset of premature senescence (Mack et al, 2005; Welford et al, 2010; Young et al, 2008). Consistent with these findings, lentiviral‐mediated shRNA knockdown of Vhl led to rapid loss of proliferative capacity of wild‐type MEFs (Fig 1A). In contrast, Vhl knockdown had no anti‐proliferative effect on Trp53−/− MEFs (Fig 1C). Interestingly, while efficient reduction of pVHL protein was maintained throughout the duration of the experiment in wild‐type cells (Fig 1B), the extent of knockdown rapidly diminished with increasing passage in Trp53−/− cells (Fig 1D), suggesting that there is a proliferative selection for those cells in which the knockdown occurs less efficiently. To attempt to overcome this problem of selection, MEFs from wild‐type, Vhlfl/fl, Trp53fl/fl and Vhlfl/flTrp53fl/fl mice were infected with adenovirus expressing GFP (Adeno‐GFP) as control or with adenovirus expressing a Cre‐GFP fusion protein (Adeno‐Cre) to delete the floxed genes. Vhl knockout MEFs rapidly lost proliferative capacity whereas Vhl/Trp53 double deletion allowed proliferation, albeit at a slower rate of proliferation than Trp53 deletion alone (Fig 1E). Consistent with a rescue of senescence, Vhl/Trp53 double null cells retained the appearance of proliferating cells while Vhl deletion caused an enlarged, flattened cell morphology characteristic of senescence (Supporting Information Fig 1). None of the gene deletions allowed the formation of colonies in soft agar, demonstrating that the cells are immortalized but not transformed. Western blotting 4 days after infection confirmed the high efficiency of gene deletion but after approximately 20–30 days of continuous proliferation, a significant restoration of pVHL expression was evident in the Vhlfl/flTrp53fl/fl Cre‐infected cell population (Fig 1F), suggesting a mixed cell population in which Vhl null cells have a proliferative disadvantage in comparison to an initially rare population of pVHL‐expressing cells (see results below).
In light of recent findings showing that senescence induced by loss of Vhl can be rescued by culturing cells at 2 or 5% oxygen (Welford et al, 2010), we conducted a series of experiments in which cells were grown at 5% oxygen. We observed a reproducible but only partial restoration of proliferative capacity following Vhl knockout (Fig 1G) when cells were cultured either in a glove box incubator to ensure constant oxygen tension throughout the experiment or in a conventional oxygen incubator where cells were briefly exposed to 21% oxygen every 3 days during passaging. In either incubator, wild‐type MEFs did not enter senescence within 50 days of culture at 5% oxygen but typically entered senescence after about 10–12 days at 21% oxygen, verifying our culture system against published data (Parrinello et al, 2003). In light of this partial rescue of proliferation of Vhl mutant cells, all subsequent proliferation experiments were conducted at 5% oxygen in a conventional incubator.
To permit comparison of proliferation rates in an isogenic background, we knocked down Trp53 in Vhlfl/fl MEFs. Trp53 knockdown rescued the proliferation defect of Vhl knockout (Adeno‐Cre treated) cells but these cells proliferated more slowly than control (Adeno‐GFP treated) cells with Trp53 knockdown alone (Fig 1I). The Vhl knockout/Trp53 knockdown cultures became enriched with pVHL‐expressing cells over time (Fig 1J). A similar reduction in proliferation rate (Fig 1K) and passage‐dependent enrichment of pVHL‐expressing cells in the cell population (Fig 1L) was also observed in cultures where Vhl was deleted from Vhlfl/fl MEFs that had been transformed with SV40 Large T‐Antigen to simultaneously inactivate both the p53 and pRB‐dependent cell cycle checkpoints. Thus, loss of pVHL compromises cellular proliferation in MEFs in a manner independent of the p53 and pRB cell cycle checkpoints.
Given the strong selection against pVHL‐expressing cells in bulk population experiments, we performed experiments using single cells to definitively address the question of whether Vhl/Trp53 double null cells are truly immortalized. While wild‐type MEFs undergo cellular senescence when plated as single cells, Trp53 null cells form colonies allowing the generation of immortalized cell lines founded from single cells. Two days after infection of Vhlfl/flTrp53fl/fl primary MEFs with Adeno‐Cre, cells were plated at a density of 0.5 cells/well in six 96‐well plates. Cell lines were generated over a period of 6 weeks and genotyped to detect the floxed or deleted Vhl allele, allowing a retrospective assessment of the genotype of the initiating cell of the cell line. From a theoretical maximum of 288 cell lines, 135 cell lines were generated. One hundred and thirty‐three of these harboured homozygous deletion of Vhl, while two were heterozygous for the floxed and deleted allele (Fig 1M). All cell lines showed homozygous deletion of the floxed Trp53 gene (Fig 1M). Western blotting of a subset of these cell lines confirmed the PCR genotyping results (Fig 1N). Thus, Trp53 deletion efficiently allows immortalization of Vhl null MEFs. It is likely that the rare cells in which only one floxed Vhl allele (but both floxed Trp53 alleles) has undergone Cre‐mediated recombination have a proliferative advantage over the Vhl/Trp53 null cells, allowing them to accumulate over time in bulk populations.
Trp53 mutation rescues proliferation of Vhl mutant primary renal epithelial cells
To investigate the cooperative effects of combined Vhl and Trp53 deletion in a disease‐relevant cell type we cultured primary mouse renal epithelial cells from the various floxed mouse strains at 5% oxygen and deleted Vhl and/or Trp53 using Adeno‐Cre or using Adeno‐GFP as control (Fig 2A). While long‐term assays of renal epithelial cell behaviour are not possible due to the epithelial to mesenchymal transition that occurs over time, in short term assays we observed that deletion of Vhl inhibited the proliferation of renal epithelial cells and co‐deletion of Trp53 rescued this inhibition of proliferation (Fig 2B). Unlike in MEFs, cultures of Trp53 or Vhl/Trp53 null renal epithelial cells formed colonies when plated at single cell density with very low efficiency (<1%), and did not grow in soft agar, demonstrating that these cells are not immortalized or transformed.
Enhanced rates of aneuploidy in Trp53 and Vhl/Trp53 mutant MEFs
One driving force in the evolution of tumours is aneuploidy. Loss of pVHL, through an uncharacterized mechanism, results in lower levels of the mitotic checkpoint protein Mad2, which alone causes a moderate elevation of levels of aneuploidy but when combined with reduction in expression of another mitotic spindle checkpoint protein, CENP‐E, induces a dramatic increase in aneuploidy (Thoma et al, 2009). Here we confirm that deletion (Fig 3A) or knockdown (Fig 3B) of Vhl causes a reduction of Mad2 protein expression. Loss of Trp53 function in MEFs also causes higher levels of aneuploidy and polyploidy and has been shown to result in aberrantly elevated expression levels of the checkpoint proteins Aurora A (Mao et al, 2007), Mad2 and separase (Pati et al, 2004). Western blotting of Trp53fl/fl cells infected with Adeno‐Cre (Fig 3A) revealed elevated expression levels of Aurora A and Mad2, as well as elevated expression of BubR1, another spindle checkpoint protein, but no change in the expression levels of CENP‐E. To our knowledge this is the first report of this effect of p53 on BubR1. Since double mutation of Mad2 and Trp53 has been shown to lead to dramatic levels of aneuploidy (Burds et al, 2005), we investigated whether the combined effects of loss of Vhl and Trp53 on the expression of various mitotic spindle checkpoint proteins would have a similar effect. However, Vhl/Trp53 double knockout (Fig 3A) or double knockdown (Fig 3B) cells displayed higher than normal levels of Mad2. This was presumably due to the elevation in mRNA abundance of Mad2 in Trp53 and Vhl/Trp53 knockout MEFs (Fig 3C), consistent with previous observations that p53 represses Mad2 mRNA expression (Pati et al, 2004), overriding the effect of loss of Vhl in reducing Mad2 expression. Thus, in terms of the expression of several proteins whose levels regulate spindle checkpoint function, Vhl/Trp53 double null cells are similar to Trp53 null cells. Functional studies supported this idea. Flow cytometry revealed that cultures of Trp53 null MEFs accumulated polyploid cells at the same frequency as cultures of Vhl/Trp53 double null MEFs (Fig 3D). To directly monitor the integrity of the mitotic spindle checkpoint in an isogenic background we performed lentiviral‐mediated knockdown of Vhl and/or Trp53 in MEFs and performed fluorescence microscopy to detect aberrant anaphases that are characterized by the presence of lagging or unattached chromosomes or DNA bridges (Fig 3E). Knockdown of Vhl led to a slightly increased rate of aberrant anaphases (Fig 3F), Trp53 knockdown and Vhl/Trp53 double knockdown both led to a statistically significant increase in the frequency of aberrant anaphases in comparison to control knockdowns, but the two genotypes were not significantly different from one another (Fig 3F).
In summary, while there appear to be no cooperative genetic effects of loss of Vhl and Trp53 function on aneuploidy, Trp53 mutation in a Vhl mutant background may enhance aneuploidy, which may be relevant for tumourigenesis.
Deletion of Vhl and Trp53 in mouse kidney and genital–urinary tract epithelia causes dysplasia and tumour formation
To investigate the consequences of combined deletion of Vhl and Trp53 in epithelial tissues in vivo, Vhlfl/fl and Trp53fl/fl mice were interbred with Ksp1.3‐Cre transgenic mice to generate Ksp1.3‐Cre; Vhlfl/fl (Frew et al, 2008b), Ksp1.3‐Cre; Trp53fl/fl (Wild et al, 2012) and Ksp1.3‐Cre; Vhlfl/fl; Trp53fl/fl mice, hereafter referred to as VhlΔ/Δ, Trp53Δ/Δ and VhlΔ/ΔTrp53Δ/Δ mice respectively. In the kidney, the Ksp1.3‐Cre transgene induces gene deletion in the epithelial cells at the urinary pole of the glomerulus, distal tubules, loops of Henle, collecting ducts and also very infrequently in proximal tubular cells. Expression of this transgene in the Wolffian and Müllerian ducts during development also leads to gene deletion in the epithelia of the renal pelvis, ureter, vesicular glands, epididymis, vas deferens and endometrium.
VhlΔ/ΔTrp53Δ/Δ mice were sub‐viable, with approximately 25% of mice dying within the first 3 months of life and with subsequent deaths in an apparently stochastic manner as the mice aged. Autopsy of these mice failed to reveal any obvious cause of death and no tumours were evident in any of the dead mice. This fact complicated the accrual of large cohorts of aged mice. Nonetheless, in combination with previously published analyses (Frew et al, 2008a, b) we analysed cohorts of mice at the following ages: 2–3 months (VhlΔ/Δ, n = 8; Trp53Δ/Δ, n = 7; VhlΔ/ΔTrp53Δ/Δ n = 6), 4–8 months (VhlΔ/Δ, n = 6; Trp53Δ/Δ, n = 10; VhlΔ/ΔTrp53Δ/Δ n = 10) and 11–13 months (VhlΔ/Δ, n = 9; Trp53Δ/Δ, n = 10; VhlΔ/ΔTrp53Δ/Δ n = 17). Littermate mice that were negative for the Ksp1.3‐Cre transgene served as controls for all of these cohorts.
As previously described, kidneys of Trp53Δ/Δ mice developed normally and showed no histological abnormalities within 18 months of age (Wild et al, 2012). Similarly to VhlΔ/Δ mice (Frew et al, 2008b), VhlΔ/ΔTrp53Δ/Δ mice developed a hydronephrosis phenotype of unknown cause but otherwise showed no defects in the structure of the nephrons at early ages. Mutation of Trp53 in combination with Vhl led to a similar accumulation of nuclear HIF1α and HIF2α in tubular epithelia to that seen in Vhl single mutant mice (Supporting Information Fig 2). By 5 months of age small clusters of disorganized cells (Fig 4F) or micro‐cysts (not shown) could infrequently be observed in the double knockout mice but not in either of the single mutant mice or control mice, suggestive of a breakdown in normal proliferative control in these cells. In comparison to the normal histological appearance of kidneys from 11‐ to 13‐month‐old control and single Vhl and Trp53 mutant mice, kidneys of 13 out of 17 VhlΔ/ΔTrp53Δ/Δ mice aged 11–13 months mice displayed multiple hyperproliferative lesions (Fig 4G) and mild focal lymphoplasmacellular inflammation. Sections through the midline of 24 kidneys from these mice revealed 399 cysts ranging in diameter from 100 µm to 1 mm. Three hundred and forty‐nine of these were lined by a single layered cuboidal epithelium (simple cyst) (Fig 4H) while 50 cysts showed multilayered micro‐papillary epithelial growths projecting into the lumen (atypical cyst) (Fig 4I). Some larger cysts showed signs of regression, bleeding, cholesterol accumulation and foam cell macrophage infiltration. An additional 16 neoplastic lesions (diameter 250 µm to 1 mm) were also observed (Fig 4J and K). These lesions were non‐invasive, displayed an increased mitotic index, low nuclear grade (Fig 4L) and cells grew either in a micro‐papillary (Fig 4J) or solid (Fig 4K and L) growth pattern. Tumour cells typically showed weak cytoplasmic eosin staining (Fig 4L), similar to, but to a lesser extent than, the clear cell morphology seen in human ccRCC. Approximately half of the neoplasms were growing into a cystic space (Fig 4J) whereas the other lesions presented as a solid mass (Fig 4K and L). It was not possible to distinguish whether these latter lesions may represent completely filled cysts or whether they have arisen as a cyst‐independent neoplasm. Epithelial cells lining simple and atypical cysts (Fig 4N and O) and neoplastic cells (Fig 4P) displayed frequent labelling for the proliferation marker Ki67. Simple cysts, atypical cysts and neoplasms all displayed high nuclear immunoreactivity for HIF1α and HIF2α (Fig 4Q–T) verifying that these lesions are derived from Vhl null cells. While it is not possible to assay for loss of p53 protein by immunohistochemistry due to the fact that p53 is not detectable in normal kidney cells, PCR genotyping of laser capture micro‐dissected simple cysts, atypical cysts and neoplasms demonstrated that the recombined Trp53 and Vhl alleles were present in cells in these lesions (Supporting Information Fig 3). The non‐recombined Trp53 floxed and Vhl floxed alleles were also detected, likely due to presence of wild‐type (Vhlfl/fl;Trp53fl/fl) stromal, inflammatory or vascular cells in these lesions.
Immunohistochemical staining using antibodies against NaPi2 (proximal), NCC (distal), THP (thick ascending loop of Henle) and AQP2 (collecting ducts) to mark different tubule segments revealed that most simple cysts express one of these markers (Supporting Information Fig 4), demonstrating that cysts arise from different nephron segments. Very rarely, remnants of the glomerulus could be observed in simple cysts (data not shown), suggesting that these cysts had arisen from the tubular epithelium at the urinary pole of the glomerulus. However, atypical cysts and neoplasms were always negative for all of the tubular markers (Supporting Information Fig 4), preventing assessment of the tubular segment of origin of these lesions and suggesting that the transition to tumour formation involves some degree of de‐differentiation. Unlike the findings reported for some precursor lesions in human VHL patient kidneys (Esteban et al, 2006), Vhl mutant cystic lesions and neoplasms in the mouse retain expression of the epithelial marker E‐cadherin and do not display the mesenchymal marker vimentin (Supporting Information Fig 5).
Thus, Vhl and Trp53 double deletion does not automatically cause proliferative dysregulation of kidney epithelial cells in vivo but eventually leads to the evolution of lesions that appear to follow a pathway of simple cyst to atypical cyst to neoplasm that is similar to the proposed disease progression model in kidneys of patients with an inherited VHL mutation. Given the apparent morphological similarities and overlapping spectrum of development of atypical cysts and neoplasms, these lesions were grouped together and considered as being distinct from simple cysts in the analyses in the remainder of this study.
VhlΔ/ΔTrp53Δ/Δ mice also displayed a variety of dysplasias and tumours in the genital–urinary tract. Deletion of Trp53 alone caused a moderate disorganisation of the epithelia in epididymal tubules, predominantly in tubules of the corpus and cauda of the epididymis, with an age‐dependent accumulation of aberrant nuclei and multi‐nucleated cells (Wild et al, 2012, Supporting Information Fig 6E). Epididymides from VhlΔ/ΔTrp53Δ/Δ mice appeared externally normal in the first months of life (Supporting Information Fig 6B) but histological analysis of aged cohorts revealed that they displayed a qualitatively more severe phenotype of nuclear abnormalities than the Trp53Δ/Δ mice (Supporting Information Fig 6F). At 11–13 months of age, the epididymides of all male VhlΔ/ΔTrp53Δ/Δ mice, but not of control or single mutant mice, displayed benign growths (Supporting Information Fig 6H). These growths were predominantly due to squamous metaplasia (Supporting Information Fig 6I) and extensive epithelial dysplasia (Supporting Information Fig 6J). These lesions are histologically identical to those arising in VhlΔ/ΔPtenΔ/Δ mice (Frew et al, 2008a). Epididymides also frequently displayed fibrosis, inflammation, foreign body reactions and metaplastic stromal changes, likely as a result of the blockage of tubules by dysplasia and squamous metaplasia. One mouse developed an epididymal clear cell papillary cystadenoma (Supporting Information Fig 7A) that appeared histologically identical to the cystadenomas that arise at high frequency in patients with an inherited VHL mutation. Vesicular glands of VhlΔ/ΔTrp53Δ/Δ mice (Supporting Information Fig 6L), but not of VhlΔ/Δ or Trp53Δ/Δ mice (not shown), were malformed. In contrast to the normal single layered epithelium, vesicular glands of VhlΔ/ΔTrp53Δ/Δ mice displayed a disorganized epithelium characterized by multiple convoluted layers of epithelial cells and the formation of gland‐like structures (Supporting Information Fig 6P). This phenotype increased in severity with age and two mice exhibited carcinomas in the vesicular gland (Supporting Information Fig 7C). The uterus in all genotypes developed normally (Supporting Information Fig 6R) and displayed a normal organisation of lumenal and glandular endometrial epithelium (Supporting Information Fig 6V). In older VhlΔ/ΔTrp53Δ/Δ mice, small foci of disorganized and multilayered epithelial cells could frequently be observed. Consistent with this, one mouse developed a high‐grade carcinoma of the endometrium (Supporting Information Fig 7E) and another a high‐grade squamous carcinoma of the upper cervix (Supporting Information Fig 7G). One mouse displayed a high‐grade carcinoma that most likely arose in the urothelium of the renal pelvis and which had also metastasized to the lungs and liver (Supporting Information Fig 7I).
Collectively, these findings demonstrate that mutation of the Vhl and Trp53 tumour suppressor genes ultimately causes dysregulation of epithelial cell proliferation and the evolution of dysplastic and malignant lesions in multiple tissues in mice.
Cooperating pathways in tumour formation in Vhl/Trp53 double mutant mice
Since we have previously shown a connection between loss of the primary cilium and cyst formation in VHL disease (Frew et al, 2008b; Thoma et al, 2007), we examined whether epithelial cells lining simple cystic lesions that arise in Vhl/Trp53 double mutant mice displayed a similar loss of primary cilia. Visualising the primary cilium using an antibody against acetylated tubulin revealed that only 40% of cystic epithelial cells but almost 90% of cells in non‐cystic tubules displayed a primary cilium (Fig 5A and B). Since only non‐proliferating cells exhibit a primary cilium we asked if this reduction in cilia frequency was simply due to the increased proliferation of cystic epithelial cells by staining for Ki67, which labels proliferating cells in all cell cycle stages. On average, 18% of cystic epithelial cells stained positively for Ki67 (Fig 5C), a far lower frequency than the frequency of cells lacking a cilium. Indeed, dual colour immunofluorescence staining experiments revealed that many Ki67 negative cystic cells lacked primary cilia (Fig 5A) demonstrating that the loss of the primary cilium is likely a consequence of loss of pVHL and not an indirect consequence of cellular proliferation and might therefore be causal to cyst formation. Neoplasms displayed a mixed phenotype with respect to cilia, some displayed a very high frequency of ciliated cells (Fig 5D), some displayed an intermediate frequency (Fig 5E) and some were almost completely devoid of cilia (Fig 5F).
We have previously demonstrated that one pathway to cilia loss involves both inactivation of pVHL and inhibition of GSK3β (Frew et al, 2008b; Thoma et al, 2007), which can occur via hyperactivation of the PI3K signalling pathway (Frew et al, 2008b). However, immunohistochemical staining using antibodies against phospho‐Thr37/46‐4E‐BP1 (P‐4EBP1) (Fig 6G–I) and phospho‐Ser240/244‐ribosomal S6 protein (P‐S6) (Fig 6J–L), two sensitive and robust downstream markers of activation of the PI3K‐mTORC1 signalling pathway, revealed that only about 6–8% of simple cysts displayed mTORC1 pathway activation above levels seen in histologically normal tubules in the same mice (Fig 6P). Interestingly, atypical cysts and neoplasms were almost always strongly positive for both of these markers (Fig 6P). Approximately half of all simple cystic lesions and almost all atypical cysts or neoplasms displayed elevated levels of the pro‐proliferative Myc protein (Fig 6M–P). Thus, multilayered or papillary growth of Vhl/Trp53 mutant cells into the lumen of cysts or growth as a solid neoplasm correlates with the acquisition of the pro‐proliferative signature of mTORC1 activation and Myc expression.
We show that TP53 is mutated in a subset of sporadic human ccRCCs and demonstrate genetically that Trp53 mutation allows Vhl null MEFs to escape senescence and proliferate in an immortalized manner. We also show that combined deletion of Vhl and Trp53 in mice results in the formation of simple and atypical cysts, as well as neoplastic lesions in kidneys and causes tumours to form in other genital tract tissues.
The long latency of tumour formation observed in mice (1 year) is consistent with our primary cell culture data showing that Vhl/Trp53 mutation causes immortalization but not transformation of MEFs. These findings clarify previous contradictory reports concerning the role of p53 in regulating senescence following loss of Vhl in mouse fibroblasts (Welford et al, 2010; Young et al, 2008). Vhl null cells nonetheless exhibit a lower proliferation rate than Vhl wild‐type cells, even in the background of loss of the p53 and pRB cell cycle checkpoints, implying that there may be additional cellular responses that represent barriers that prevent full transformation of Vhl/Trp53 mutant cells. We suggest that the increase in aneuploidy observed in Vhl/Trp53 null MEFs might potentially represent a mechanism that could contribute to cellular transformation and tumour evolution in vivo.
Our findings strengthen the model derived from studies of human VHL patients that ccRCCs can form via cyst‐dependent and cyst‐independent pathways (Fig 6Q). Vhl/Trp53 mutant mice develop an apparent spectrum of cystic lesions beginning with simple cysts lined by a single layer of epithelial cells, followed by atypical cysts that display micro‐papillary epithelial growths that project into the lumen of the cyst and finally cysts that are almost entirely filled with neoplastic growth. About half of the neoplasms are a solid mass of cells, preventing assessment of whether they arise via a cystic precursor lesion or not. Vhl/Trp53 neoplastic lesions display several features of human ccRCC including clear cell‐like changes, HIFα stabilisation and high rate of proliferation, but differ in that they exhibit a low nuclear grade and do not invade surrounding tissue. The lack of a capsule surrounding the neoplasms and absence of extra‐renal metastases speaks against a malignant ccRCC lesion. Vhl/Trp53 mutant neoplasms also frequently grow in a micro‐papillary pattern, akin to papillary renal cell carcinomas. While the precursor lesions of human renal carcinomas are poorly characterized in general, in papillary type I and type II tumours the size of the lesion is the sole definitive distinguishing criteria. Lesions smaller than 5 mm are classified as adenomas and larger lesions are carcinomas (Eble et al, 2004). Taking the relative sizes of the human and mouse kidney into account, many of the neoplasms in our model would be classified as carcinomas under this definition. Because of the mixed features of the Vhl/Trp53 null neoplasms we classify these tumours simply as renal neoplasms, rather than as a specific sub‐type of renal cell carcinoma.
Epithelial cells lining simple cystic lesions display a reduced frequency of primary cilia, similar to cysts in human VHL patients (Thoma et al, 2007), further supporting the involvement of pVHL in maintenance of primary cilia and suppression of cyst formation. However, in contrast to Vhl/Pten mutant mice (Frew et al, 2008b), in Vhl/Trp53 mutant mice, these simple cysts do not display evidence of over‐activation of the PI3K signalling pathway or inactivating phosphorylation of GSK3β (unpublished observations), implying that there may be other unidentified pathways that cooperate with pVHL in maintenance of the primary cilium. In contrast to simple cysts, atypical cysts and neoplasms display hyperactivation of mTORC1 signalling. Since both lesions are characterized by disorganized patterns of cellular growth it is noteworthy that mTORC1 activation has been shown to induce a translational program that promotes cellular invasion (Hsieh et al, 2012). Hyperactivation of mTORC1 predicts poor outcome in ccRCC patients and mTORC1 inhibitors show clinical efficacy against ccRCC (Hudes, 2009). Atypical cysts and neoplasms almost invariably also display high levels of Myc protein. Upregulation of MYC expression is common in ccRCC and amplification of MYC predicts poor outcome in human ccRCC patients (Monzon et al, 2011; Tang et al, 2009). The combination of Vhl/Trp53 double mutation with a pro‐proliferative signature of mTORC1 activation and high Myc expression therefore correlates with the transition to a neoplastic state.
While approximately 1 in 10 ccRCC tumours harbour TP53 mutations, in many epithelial malignancies the TP53 mutation frequency is much higher (50–90%). In ccRCC, several mechanisms have been proposed to act to compromise p53 function, potentially alleviating the selective pressure for TP53 mutation or deletion during tumour formation. USP10 normally de‐ubiquitinates p53 in response to DNA damage, opposing the action of Mdm2 and allowing p53 protein accumulation (Yuan et al, 2010). Interestingly, 90% of ccRCC express lower than normal levels of USP10, possibly leading to reduced p53 activation (Yuan et al, 2010). pVHL itself has been implicated as a factor important for full p53 activation by promoting the recruitment of the p300 acetylase and ATM kinase to p53 (Roe et al, 2006). Knockdown of VHL expression reduced p53 activity in response to DNA damage and reintroduction of pVHL expression in VHL‐deficient RCC cells enhanced damage‐induced activation of p53 (Roe et al, 2006). Downstream of loss of pVHL function, activation of HIFα transcription factors may also act to compromise p53 activity. The hypoxia‐inducible PAX2 gene is a transcriptional repressor of TP53 and is highly upregulated in VHL mutant cells and ccRCCs (Luu et al, 2009; Stuart et al, 1995). Elevated HIF2α levels in VHL‐mutant ccRCC are proposed to induce growth factor expression leading firstly to the AKT‐mediated phosphorylation of HDM2, promoting its ability to degrade p53 (Roberts et al, 2009) or secondly to the suppression of formation of reactive oxygen species which reduce p53 activation (Bertout et al, 2009). PBRM1 is mutated in 41% of ccRCCs (Varela et al, 2011) and has been shown to be necessary for induction of senescence by p53 (Burrows et al, 2010), thus potentially abrogating part of p53's tumour suppressing activity in the kidney. In our hands however, knockdown of Pbrm1 failed to alleviate proliferative arrest following Vhl knockout in MEFs (unpublished observations). Similarly, SETD2 is mutated in a small fraction of ccRCCs (Dalgliesh et al, 2010) and has been suggested to regulate a subset of p53 target genes (Xie et al, 2008). Thus, p53 function may either be lost by mutation or compromised by other mechanisms in a large proportion of VHL‐negative ccRCCs.
It will be important to clarify when TP53 mutations arise during the process of tumour initiation and progression. In this regard, a study of four ccRCCs utilized deep sequencing of the tumour DNA population to reconstruct the molecular evolutionary history of the tumours (Gerstung et al, 2012). In one of these tumours a single TP53 truncation mutation was present at about one‐fifteenth the frequency of a single VHL frameshift mutation, implying that the TP53 mutation was an event that occurred secondarily to an initiating VHL mutation and that it resulted in the formation of a VHL/TP53 double mutant sub‐clone of the tumour cell population. This finding supports the notion that genetic cooperation between VHL and TP53 mutations promotes tumour progression. Similar analyses of larger numbers of ccRCC samples from different stages of disease progression would test how representative this initial finding is for ccRCCs in general.
In summary, we present strong evidence to support the idea that loss of function of VHL and TP53 is a bone fide tumour promoting combination and describe a mouse model that recapitulates many of the steps involved in the formation of VHL mutant kidney tumours in humans.
MATERIALS AND METHODS
Previously described Ksp1.3‐Cre/+; Vhlfl/fl (Frew et al, 2008b) and Ksp1.3‐Cre/+; Trp53fl/fl (Wild et al, 2012) mouse strains were interbred to generate Ksp1.3‐Cre/+; Vhlfl/fl; Trp53fl/fl mice. Non‐Cre transgenic littermate mice served as controls for all cohorts. Wild‐type cells were isolated from C57BL/6 embryos.
Analyses of human ccRCCs
Tissue samples were from the University Hospital of Zurich (Zurich, Switzerland). The study was approved by the local ethics commission (reference number StV 38‐2005). Haematoxylin and eosin stained sections of all paraffin embedded ccRCC specimens were reviewed by H.M. DNA extraction and VHL sequencing were performed as previously described (von Teichman et al, 2011). The primers used for PCR and sequencing of TP53 exons 5–8 are listed in Supporting Information Table S1. PCR was performed with 40 cycles consisting of denaturation at 94°C for 45 s, annealing at 58°C for 45 s and extension at 72°C for 45 s. VHL and TP53 mutations were validated by an independent PCR and sequence analysis. Paraffin sections (2.5 µm) were treated using Ventana Benchmark XT (Tuscon, AZ, USA) or BOND‐MAX (Leica Microsystems, Wetzlar, Germany) automated systems. Immunostainings for CAIX, GLUT1 and HIF1α were performed as recently described (Dahinden et al, 2010; Luu et al, 2009). Nuclear HIF1α and membranous CAIX, GLUT1 expression were defined positive if at least 5% of tumour cells showed weak (+1) or strong (+2) staining.
Assays of MEFs
MEFs were isolated from relevant floxed strains and aliquots were frozen at passage 2. Trp53−/− MEFs were a kind gift from Scott Lowe. Cells were cultured either in conventional cell culture incubators at atmospheric oxygen or at 5% oxygen or were cultured in a darkened oxygen glove‐box incubator (INVIVO2 400, Ruskinn) at 5% oxygen in which medium and PBS were equilibrated for 2 h prior to splitting of cells to ensure that cells were exposed to constant oxygen tension throughout the experiment. For proliferation assays, cells were seeded at densities of either 2 × 105 or 3 × 105 cells per 6 cm dish in triplicate dishes and counted after 3 days before reseeding at the same density for the next passage. All proliferation assays shown in the Figures are representative of at least three independent experiments. Wild‐type and Vhlfl/fl MEFs were transformed by transfection with a plasmid expressing SV40 large T‐Antigen (Addgene, pBSSVD2005) and pools of cells that formed colonies after plating at low density were harvested to generate cell lines. Cells were infected with adenoviruses expressing GFP (Vector Biolabs, 1060) or Cre‐GFP (Vector Biolabs, 1700), retroviruses (LMP) expressing non‐silencing hairpin or miR30‐shRNA against Trp53 (Dickins et al, 2005), lentiviruses (LKO.1) expressing non‐silencing hairpin (Addgene, 10879) or shRNA against Vhl (Open Biosystems, TRC0000009735) (Thoma et al, 2007). For lentiviral‐mediated knockdown of Trp53, we generated a vector (pLenti X1 Puro DEST, Addgene 17297) containing the U6 promoter (derived from pENTR/pSM2 (U6), Addgene 17387) driving expression of a previously described (Dickins et al, 2005) miR30 format shRNA against Trp53 (1224) or expressing an empty (ns) miR30 backbone. Infections were followed after 48 h by puromycin selection (4 µg/ml) where appropriate. Genotyping for the floxed or recombined Vhl and Trp53 alleles were performed as described (Biju et al, 2004; Jonkers et al, 2001). Flow cytometry (Frew et al, 2002) and counting of aberrant anaphases (Burds et al, 2005) were performed as described.
Real‐time PCR was performed as described (Frew et al, 2008b) using the following primer pairs: 18S rRNA (5′‐TGGCCGACCATAAACGATGCC‐3′, 5′‐TGGTGGTGCCCTTCCGTCAAT‐3′), Mad2 (5′‐GTGGCCGAGTTTTTCTCATTTG‐3′, 5′‐AGGTGAGTCCATATTTCTGCACT‐3′).
Kidney epithelial cell proliferation assays
Kidneys were dissected from 2‐month‐old floxed mice. After removing the capsule under sterile conditions, kidneys were mashed with a razor blade on ice and digested in collagenase II (Gibco) and soya trypsin inhibitor (Gibco) solution at 37°C for 30 min. The cell suspension was filtered through a 70 µm cell strainer and washed in HBSS + 5% FCS. Erythrocytes were lysed for 1 min using standard ACK buffer. Cells were resuspended in complete K‐1 culture medium [Dulbecco's modified Eagle's medium (DMEM):Hams F12] (50:50), supplemented with 0.5% foetal calf serum, hormone mix [5 µg/ml insulin, 1.25 ng/ml prostaglandin E1 (PGE1), 34 pg/ml triiodothyronine, 5 µg/ml Apo‐transferrin, 1.73 ng/ml sodium selenite and 18 ng/ml of hydrocortisone] and 25 ng/ml epidermal growth factor (EGF). Cells were counted and seeded at a density of 1 × 106 cells on standard 100 mm plastic tissue culture plates. After 5–6 days in culture, cells were infected with adenoviruses expressing GFP (Vector Biolabs, 1060) or Cre‐GFP (Vector Biolabs, 1700). Sulforhodamine B (SRB) proliferation assay was performed in 96‐well format as described (Vichai & Kirtikara, 2006). Briefly, primary kidney epithelial cells were cultured in K‐1 medium containing 10% foetal calf serum for 2 days before seeding for the SRB assay. 2 × 103 cells per well were seeded and fixed in 5% w/v trichloracetic acid at the indicated time points. Cells were stained in 0.057% w/v SRB solution and air dried. SRB was solubilized by incubation in 10 mM Tris base solution (pH 10.5) and OD was measured at 540 nm in a micro‐plate reader.
Antibodies, Western blotting, immunofluorescence and immunohistochemistry
Western blotting, immunohistochemistry or immunofluorescence were conducted using previously described methods (Frew et al, 2008b) and the antibodies against the following epitopes: Acetylated tubulin (Sigma, #T6793), Actin (Sigma‐Aldrich, A2228), AQP2 (Wagner et al, 2008), Aurora A (Abcam, ab13824), BubR1 (BD Biosciences, 612502), CDK‐2 (Santa Cruz, sc‐163‐g), Cenp‐E (Meraldi et al, 2004), E‐cadherin (Abcam, ab11512), phospho‐Thr37/46‐4E‐BP1 (Cell Signaling Technology, #2855), HIF1α (Novus Biologicals, NB100‐105), HIF2α (Pollard et al, 2007, PM8), Ki67 (DakoCytomation, TEC‐3), Mad2 (Bethyl Laboratories, A300301A), Myc (Epitomics, Y69), p53 (Novocastra, NCL‐p53‐CM5p), NaPi2 (Custer et al, 1994), NCC (Millipore, AB3553), phospho‐Ser240/244‐ribosomal S6 protein (Cell Signaling Technology, #2215), THP (Santa Cruz Biotechnology, sc‐20631), pVHL(m)CT antibody (Hergovich et al, 2003), pVHL (Santa Cruz, sc‐5575), Vimentin (Cell Signaling Technology, #5741).
For more information
For information about the VHL Family Alliance for patients with inherited VHL disease:
For general information about kidney cancer subtypes and treatments:
For information about the genetic mutations that have been found in ccRCC:
IJF and WK designed the study, IJF, JA, MR, DS, SH, PS, AvT and SG conducted and analysed the experiments, PJW and HM performed histopathological analyses and the manuscript was written by IJF with the assistance of all authors.
As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re‐organized for online delivery, but are not copy‐edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors.
This work was supported by grants to I.J.F. from SNF Förderungsprofessur (PP00P3_128257) and ERC Starting Grant (260316), to J.A. from the European Community's Seventh Framework Programme (FP7/2007‐2013) under grant agreement no. 246539, and to W.K. from the SNF. We are grateful to Johannes Loffing and Jürg Biber for providing antibodies and to the Centre for Microscopy and Imaging Analysis, University of Zurich for assistance with laser capture microdissection.
Supporting Information is available at EMBO Molecular Medicine online.
The authors declare that they have no conflicts of interest.
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