Split otoferlins reunited

Split AAVs, each containing a fragment of the large otoferlin coding sequence are injected into the inner ears of otoferlin knockout mice. Upon co‐transduction in single cells, the split sequences reassemble full‐length otoferlin, which in sensory hair cells leads to recovery of protein expression, partial recovery of synaptic function and auditory brainstem responses (ABR). Bottom panels illustrate synaptic and ABR recovery after dual‐AAV treatment (orange) relative to Otof^−/− mice (blue) and wild‐type mice (black).